ผลงานวิจัย

หมายเลขหนังสือ1171
ชื่องานวิจัยThe Immunomodulating Effects of Lacquered Mushroom (Ganoderma Lucidum) on Lymphocytes of HIV-Infected Patients
ชื่อผู้แต่ง Sunee Sirivichayakul
สถาบันFaculty of Medicine, Chulalongkorn University , Bangkok Tel. 254-2323 Fax 254-0212
ที่มาSirivichayakul S., Phanuphak P. and Hanvanich M.
คำสำคัญimmunity, traditional medicine, therapeutics
ปี พ.ศ.2535
จำนวนหน้า0
ประเภทเอกสารบทคัดย่อ
บทคัดย่อObjectives : To study the immunomodulating properties of lacquered mushroom extract (Ganoderma lucidum) on the phenotypes of T cells from HIV-infected individuals. Methods : Twenty-two HIV patients : 2 full-blown AIDS, 3 ARC (AIDS-related complex), 8 PGL (persistent generalized lymphadenopathy) and 9 asymptomatic HIV carriers were included in this study. Five normal volunteers served as controls. Peripheral blood mononuclear cells (PBMC) were obtained by Ficoll-Hypaque density gradient centrifugation. Different concentrations of Bioherb (commercial lyophilized extract of Ganodema lucidum) were tested for the ability to change the T cell subset phenotypes by in vitro colultivation with PBMC for 3 days. T helper and T suppressor cells were enumerated by indirect immunofluoresoent staining with OKT4 and OKT8 monoclonal antibodies respectively. Any increase of 20% or more above background was considered of having immunomodulating effect. Results : From this study, it was shown that Bioherb at 10 mg/ml. was the most effective concentration in enhancing the expression of T4 phenotype of PBMC. It increased the number of T helper cells in 16 out of 27 cases (59.3%) whereas T suppressor cells were unaffected. The mean increase of T helper cells from HIV-infected individuals was 53+/-61.4% whereas that of uninfected individuals was 32.6+/-6.4%. Conclusions : Our preliminary data indicated that G. lucidum extract had some immunomodulating effect on T helper cells in vitro. The effect was more pronounced on HIV-infected cells than uninfected cells. It warrants a clinical trial on HIV-infected patients although one has to realize that the response may be quite variable.
 
พฤ อา
 

E-Service