ผลงานวิจัย

หมายเลขหนังสือ4
ชื่องานวิจัยFlow Cytometric Three-Color Determination of CD4 T-Lymphocytes on Blood Specimens from AIDS Patients who have a Large Number of Contaminating Non-Lymphocytes
ชื่อผู้แต่งKovit Pattanapanyasat1, Pengruangrojanachai V., Thepthai C., Suwanagool S. and Wasi C.
สถาบัน1Faculty of Medicine, Siriraj Hospital, Mahidol University
ที่มาPattanapanyasat K., Pengruangrojanachai V., Thepthai C., Suwanagool S. and Wasi C.
คำสำคัญprognosis, immunity
ปี พ.ศ.2538
จำนวนหน้า0
ประเภทเอกสารบทคัดย่อ
บทคัดย่อA Three-color flow cytometric determination of CD4 T-lymphocytes on whole blood specimens from AIDS patients which contain a high proportion of non-lymphocyte elements is describe. Peripheral blood cells were stained by a three-color method using monoclonal antibodies conjugated respectively with fluorescein isothiocyanate (FITC)-CD3, phycoerythrin (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45. CD45 stains all leukocytes with the highest fluorescence expression of CD45 antigen in lymphocytes. By combining light scatter with CD45 in the fluorescence 3 (FL) channel, a light scattering window can be drawn to include almost all bright CD45 lymphocytes. This live gate of lymphocytes was then acquired and analysed simultaneously using other irrelevant two-color (FITC/PE) antibodies of CD3 and CD4 in the FITC and PE channels, respectively. This method is easy and straightforward, and gives successful analysis of CD4 T-lymphocytes in AIDS blood specimens contaminated with an unusually large number of non-lymphoocytic cells.
 
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