หมายเลขหนังสือ | 4 |
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ชื่องานวิจัย | Flow Cytometric Three-Color Determination of CD4 T-Lymphocytes on Blood Specimens from AIDS Patients who have a Large Number of Contaminating Non-Lymphocytes |
ชื่อผู้แต่ง | Kovit Pattanapanyasat1, Pengruangrojanachai V., Thepthai C., Suwanagool S. and Wasi C. |
สถาบัน | 1Faculty of Medicine, Siriraj Hospital, Mahidol University |
ที่มา | Pattanapanyasat K., Pengruangrojanachai V., Thepthai C., Suwanagool S. and Wasi C. |
คำสำคัญ | prognosis, immunity |
ปี พ.ศ. | 2538 |
จำนวนหน้า | 0 |
ประเภทเอกสาร | บทคัดย่อ |
บทคัดย่อ | A Three-color flow cytometric determination of CD4 T-lymphocytes on whole blood specimens from AIDS patients which contain a high proportion of non-lymphocyte elements is describe. Peripheral blood cells were stained by a three-color method using monoclonal antibodies conjugated respectively with fluorescein isothiocyanate (FITC)-CD3, phycoerythrin (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45. CD45 stains all leukocytes with the highest fluorescence expression of CD45 antigen in lymphocytes. By combining light scatter with CD45 in the fluorescence 3 (FL) channel, a light scattering window can be drawn to include almost all bright CD45 lymphocytes. This live gate of lymphocytes was then acquired and analysed simultaneously using other irrelevant two-color (FITC/PE) antibodies of CD3 and CD4 in the FITC and PE channels, respectively. This method is easy and straightforward, and gives successful analysis of CD4 T-lymphocytes in AIDS blood specimens contaminated with an unusually large number of non-lymphoocytic cells. |